Fig. 2

TRPV1-ablation impairs antigen-specific antibody response following immunization. (A) TRPV1-Cre/DTA (n = 7) or flox-DTA control mice (n = 5) were immunized with 4 mg/kg KLH intraperitoneally and serum was assayed for anti-KLH IgG antibodies by ELISA. Data are represented as individual mouse data points with mean ± SEM. Ordinary one-way ANOVA with multiple comparisons test between groups: flox-DTA control versus TRPV1-Cre/DTA; **p < 0.01, ***p < 0.001. (B-C) TRPV1-Cre/DTA mice or flox-DTA control mice were also immunized with NP-OVA in the foot pad and serum collected every 7 days. (B) High affinity and (C) low-affinity NP-specific IgG1 antibodies were quantified by ELISA. Data is represented as mean ± SEM for each time point. (n = 18/group from 2 repeated experiments). Two-way ANOVA: flox-DTA control versus TRPV1-Cre/DTA; *p < 0.05