Fig. 4

TRPV1 ablation does not impair the antibody response of in vitro activated splenocytes. Splenocytes from flox-DTA control or TRPV1-Cre/DTA mice were stimulated in vitro with LPS, and the number of antibody secreting cells (ASC) was determined by ELISpot assay on anti-IgG coated plates. (A) Representative images from total IgG ELISpot assay performed on splenocytes from flox-DTA control mice or TRPV1-Cre/DTA mice. Data are representative of 3 independent experiments. (B) Quantification of ASCs in unstimulated and LPS-stimulated splenocytes from flox-DTA control and TRPV1-Cre/DTA mice using anti-IgG ELISpot assay. (C) Supernatant from unstimulated or LPS-stimulated splenocytes from flox-DTA control and TRPV1-Cre/DTA mice was collected after 72 h. Levels of total IgG secreted was measured using ELISA. Data are represented as individual data point for each experiment with mean ± SEM. Each experiment was carried out with at least 3 replicates per experiment